Assessment of DNA damage induced by butachlor on Cyprinuscarpio(L. 1758) using Single Cell Gel Electrophoresis

Authors

A. Mashinchian Moradi Department of Marine Biology, Graduate School of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

M. AlidoustSalimi Department of Marine Biology, Graduate School of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

M. H. Shahhosseini Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

P. AlidoustSalimi Department of Marine Biology, Graduate School of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract:

Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present study was undertaken to investigate of DNA damage by butachlor in Cyprinuscarpio(Pisces: Cyprinidae) using single cell gel electrophoresis. This study was done according to static method. The specimens were exposed to different concentrations of butachlor (0.07, 0.15 and 0.3 mgl-1) and sampling was carried out after 48 hour (short-time exposed). Based on the results, a significant increase in genetic damage index was observed in treatments compared with control samples (P

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Journal title

International Journal of MArine Science and Engineering

volume 2 issue 4

pages 233- 238

publication date 2012-12-19

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Keywords

Comet assay Common carp Genetic Damage Index (GDI) Genotoxicity Herbicide

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